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. 2019 Nov 25;130(1):272–286. doi: 10.1172/JCI129833

Figure 3. TSS of SLC26A9 in pancreas.

Figure 3

(A) Schematic in native orientation showing the first 5 exons of the SLC26A9 gene. Note: SLC26A9 is transcribed from the minus strand. The size of exon and intron regions are labeled (nt). Hatch marks denote where the figure is not drawn to scale. (B) Summary of sequence of 5′ RACE obtained from 1 primary human pancreas RNA. 5′ RACE was performed using a gene-specific primer (GSP) in exon 5 of SLC26A9. The portion of the GSP in red is the overhang necessary for infusion PCR. TSS marks the beginning of exon 1. The translational start site with the Kozak consensus sequence occurs in exon 2. (C) Sanger sequencing trace of the 5′ RACE product from the SLC26A9 mRNA transcripts in human pancreas. Upstream of the TSS is the RACE adapter sequence confirming the 5′ most extent of the RACE product. The sequencing trace crosses exon/exon junctions (shown here between exon 1 and 2 by the vertical black line) confirming that RACE used mRNA as the template. Sanger sequencing of 5′ RACE products obtained from primary human lung (n = 3) and stomach (n = 1) samples identified the same TSS (not shown).