Figure 6. Treatment with DON altered immune profile in orthotopic syngeneic mouse model.

In the orthotopic implantation of KPC and CAF in the pancreas of C57BL/6 mice, DON increased CD68⁺ macrophage populations (A and B). *P < 0.05. Treatment with DON also increased intratumoral CD8⁺ infiltration, as seen by flow cytometry (C) and immunohistochemistry (D). Effect of DON on tumor and stroma in syngeneic model was CD8 mediated, as pancreatic tumors implanted in CD8-KO mice did not show improved survival (E) or decrease in tumor volume (n = 3) (F) with DON. Original magnification, ×10 (A and D).