Skip to main content
. 2019 Nov 25;130(1):214–230. doi: 10.1172/JCI130711

Figure 3. CD1-restricted T cells in blood and lung during active TB.

Figure 3

(A) The frequency of iNKTs in blood (open circles) compared with lung (solid circles) among 5 clinical populations. (B) iNKT cell frequency in paired lung and blood samples, stratified by HIV status for subjects with active TB. Each line represents a single individual. (C) Using a consensus definition of GEM TCRα (Vα1-02, Jα9, CDR3α=CAV(R/L)xTGGFKTIF), GEMs were identified in lung tissue. The 6 most frequently detected clonotypes are shown, with their total relative abundance in the aggregated lung TCR data set. (D) Frequency of GEMs in blood (open circles) versus lung tissue (solid circles) among 5 clinical populations. (E) Clonotypes encoded by TRAV01-2 with the CDR3α CAV(R/L)xTGGFKTIF were identified in HCs (blood, top) and subjects in the lung cohort (blood, second graph; lung, third graph). Non–TRAV01-2 clonotypes encoding the CAV(R/L)xTGGFKTIF CDR3α were identified in lung (bottom graph). A frequency of 0.0000001 was assigned to clonotypes not detected. Dotted lines indicate a productive frequency of 0.05%. Colored violins indicate the most frequently detected lung clonotypes. Red lines indicate the median and blue lines the quartiles.