Figure 8. Gpr101 knockdown limits the ability of RvD5_{n-3 DPA} to activate host responses to promote the resolution of E. coli infections.

(A) Mice were inoculated with 10⁵ CFU E. coli via i.p. injection and lavages collected at the indicated time intervals before (0 hours) or after inoculation. RvD5_{n-3 DPA} concentrations were determined using lipid mediator profiling. Results represent the mean ± SEM (n = 3 mice per group). (B) Mice were administered 9 μg siRNA against mouse Gpr101 or a scrambled control sequence, and after 72 hours, they were administered RvD5_{n-3 DPA} (100 ng/mouse) or vehicle control (PBS containing 0.1% ethanol) and then inoculated with 10⁵ CFU E. coli via i.p. injection. (C) Fourteen-hour exudate neutrophil counts. Results represent the mean ± SEM (n = 6 mice per group from 2 distinct experiments). (D and E) Bacterial phagocytosis was determined in exudate (D) neutrophils and (E) macrophages at the 14-hour interval using flow cytometry. (F) Efferocytosis was determined at the 14-hour interval in CD64⁺F4/80⁺ macrophages using flow cytometry. (G) The expression of MHC class II and IL-10R was assessed in CD64⁺F4/80⁺ macrophages using flow cytometry. Results represent the mean ± SEM (n = 6 mice per group from 2 distinct experiments). *P < 0.05 versus the vehicle-treated group; Kruskal-Wallis test with Dunn’s post hoc multiple comparisons test (C–G).