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. 2019 Dec 27;15(12):e1008501. doi: 10.1371/journal.pgen.1008501

Fig 2. DIRC3 is a direct MITF and SOX10 transcriptional target.

Fig 2

(A) UCSC genome browser view showing that the DIRC3 locus contains multiple ChIP-seq defined binding sites for MITF and SOX10 in 501mel cells [29]. MITF (B) and SOX10 (C) repress DIRC3 in human melanoma cells. MITF and SOX10 were depleted in SK-MEL-28, 501mel and A375 cells using siRNA transfection. Expression changes were analysed using RT-qPCR. POLII was used as a reference gene. Results presented as mean +/- SEM., n≥3; one-tailed t-test * p<0.05, ** p<0.01. MITF and SOX10 protein levels are shown in S3 Fig. (D, E) DIRC3 expression inversely correlates with MITF (D) and SOX10 (E) in melanoma patients. DIRC3 levels were analysed in 471 TCGA human melanoma samples ranked using increasing MITF or SOX10. Vertical grey lines indicate DIRC3 expression in each melanoma sample. Moving averages are plotted in bold.