(A-D) Strain/mouse background comparison (accuracy) of female and male Wild-type controls (B6129SF2/J, B6SJLF1/J, C57Bl6/J) at 3–6 and 11–13 months of age; (E-H) Strain/mouse background comparison (premature responses) of female and male Wild-type controls (B6129SF2/J, B6SJLF1/J, C57Bl6/J) at 3–6 and 11–13 months of age; (I-L) Sex comparison (accuracy) of B6129SF2/J and 3xTG-AD females and males at 3–6 and 11–13 months of age. Results are presented as means ± s.e.m.; data were analysed and compared using Repeated measure Two-Way ANOVA and Bonferroni multiple comparisons post-hot test; *p<0.05, **p<0.01 and ***p<0.001 compared with control.