Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|
|---|---|---|---|---|---|
| Gene (Homo sapiens) | hsSlo1 (human_KCNMA1) | synthetic | accession: Q12791.2 GI: 46396283 |
synthesized at GeneWiz | |
| gene (Homo sapiens) | hsbeta4 (human_KCNMB4) | synthetic | accession: NP_055320.4 GI: 26051275 |
synthesized at GeneWiz | |
| gene (Homo sapiens) | hsbeta1(human_KCNMB1) | synthetic | accession: Q16558.5 GI: 292495100 |
synthesized at GeneWiz | |
| Recombinant DNA reagent | pEG BacMam | DOI: 10.1038/nprot.2014.173 | |||
| Recombinant DNA reagent | pGEM | https://www.addgene.org/vector-database/2835/ | |||
| Cell line (Homo sapiens) | HEK293S GnTI- | ATCC | CRL-3022 | cells purchased from ATCC and we have now confirmed there is no mycoplasma contamination | |
| Cell line (Homo sapiens) | HEK293T | ATCC | CRL-3216 | cells purchased from ATCC and we have now confirmed there is no mycoplasma contamination | |
| Cell line (Spodoptera frugiperda) | Sf9 | ATCC | CRL-1711 | cells purchased from ATCC and we have now confirmed there is no mycoplasma contamination | |
| Strain, strain background (Escherichia coli) | DH10Bac | ThermoFisher | 10361012 | MAX Efficiency DH10Bac Competent Cells | |
| Biological sample (Xenopus laevi) | oocyte | Xenopus laevi | |||
| Chemical compound, drug | Freestyle 293 medium | Gibco | 12338018 | ||
| Chemical compound, drug | sf-900 II SFM medium | Gibco | 10902088 | ||
| Chemical compound, drug | 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG) | Anatrace | NG310 | ||
| Chemical compound, drug | Cholesteryl hemisuccinate (CHS) | Anatrace | CH210 | ||
| Chemical compound, drug | Digitonin | Sigma-Aldrich | D141 | ||
| Chemical compound, drug | Cellfectin II | Invitrogen | 10362100 | ||
| Chemical compound, drug | FuGENE HD transfection reagent | Promega | E2312 | ||
| Chemical compound, drug | Collegenase type II | Gibco | 17107–0125 | ||
| Chemical compound, drug | Gentamicin sulphate | Sigma-Aldrich | A0752 | ||
| Commercial assay or kit | CNBr-activated sepharose beads | GE Healthcare | 17043001 | ||
| Commercial assay or kit |
Superose 6, 10/300 GL | GE Healthcare | 17517201 | ||
| Commercial assay or kit | mMESSAGE mMACHINE T7 Transcription Kit | ThermoFisher | AM1344 | ||
| Commercial assay or kit | AmpliCap-MaxT7 high yield message maker kit | CELLSCRIPT | C-ACM04037 | ||
| Commercial assay or kit | R1.2/1.3 400 mesh Au holey carbon grids | Quantifoil | 1210627 | ||
| Software, algorithm | SerialEM | DOI: 10.1016/j.jsb.2005.07.007 | http://bio3d.colorado.edu/SerialEM | ||
| Software, algorithm | MotionCor2 | DOI: 10.1038/nmeth.4193 | https://msg.ucsf.edu/software | ||
| Software, algorithm | Gctf | DOI: 10.1016/j.jsb.2015.11.003 | https://www.mrc-lmb.cam.ac.uk/kzhang/ | ||
| Software, algorithm | Gautomatch | other | https://www.mrc-lmb.cam.ac.uk/kzhang/ | ||
| Software, algorithm | cryoSPARC | DOI: 10.1038/nmeth.4169 | http://www.cryosparc.com | ||
| Software, algorithm | RELION-3 | DOI: 10.7554/eLife.18722 | http://www2.mrc-lmb.cam.ac.uk/relion | ||
| Software, algorithm | FrealignX | DOI: 10.1016/j.jsb.2013.07.005 | http://grigoriefflab.janelia.org/frealign | ||
| Software, algorithm | COOT | DOI: 10.1107/S0907444910007493 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | ||
| Software, algorithm | PHENIX | DOI: 10.1107/S2059798318006551 | https://www.phenix-online.org | ||
| Software, algorithm | UCSF Chimera | DOI: 10.1002/jcc.20084 | https://www.cgl.ucsf.edu/chimera | ||
| Software, algorithm | Pymol | PyMOL Molecular Graphics System, Schrödinger, LLC | http://www.pymol.org | ||
| Software, algorithm |
HOLE | DOI: 10.1016/s0263-7855(97)00009-x | http://www.holeprogram.org | ||
| Software, algorithm | pClamp | Axon Instruments, Inc |