Table 2. List of the β4 mutants for TEVC studies, related to Figure 4.

ShortName	β4 sequence	β1 sequence		ShortName	β4 sequence	β1 sequence
Slo1	N/A	N/A		m27	1-10,20-190,206-210	10-18,178-191
β1	N/A	1-191		m28	11-193	1-9,181-191
β4	1-210	N/A		m29	11-198	1-9,181-186
m2	7-210	N/A		m30	49-210	1-47
m3	1-205	N/A		m31	1-163	151-191
m4	10-210	N/A		m32	1-10,49-210	10-47
m5	14-210	1-12		m33	1-163,206-210	151-191
m6	1-34,40-210	34-38		m34	1-10,49-163	10-47,151-191
m7	1-190,197-210	178-183		m35	1-28,49-210	28-47
m8	1-10,14-210	10-12		m36	1-163,181-210	151-167
m9	E9A	N/A		m37	1-19,30-210	19-28
m10	1-18,20-210 (R19C)	18		m38	1-180,191-210	168-177
m11	R19L	N/A		m39	1-10,30-210	10-28
m12	E14A	N/A		m40	1-180,206-210	168-191
m13	1-42,44-210 (A43Y)	42		m41	1-10,20-210	10-18
m14	1-164,168-210	152-154		m42	1-190,206-210	178-191
m16	7-205	N/A		m43	11-210	1-9
m17	1-42,44-164,168-210	42,152-154		m44	replace β4EC (49-166) with "GGSGGGSG"	N/A
m18	1-11,13-14,16-18,20-210 (E12R, D15T, R19C)	11,14,18		m45	replace β1EC (41-153) with "GGSGGGSG"	N/A
m19	1-48,164-210	48-150		m46	replace β1EC (48-153) with "GGSGGGSG"	N/A
m20	1-45,167-210	45-153		m47	1-47,151-210	49-163
m21	49-163	1-47,151-191		m48	1-44,154-210	46-166
m22	1-10,49-163,206-210	10-47,151-191		m49	1-6,49-210	6-47
m23	1-10,49-163,196-210	10-47,151-182		m50	1-6,30-210	6-28
m24	1-28,49-163,181-210	28-47,151-167		m51	1-2,30-210	2-28
m25	1-19,30-180,191-210	19-28,168-177		m52	1-6,20-210	6-18
m26	1-10,30-180,206-210	10-28,168-191		m53	1-2,20-210	2-18