Table 2.
Rice varieties | Isolate name | Soil sampling site | Fields | Origin of isolates associated with rice | Closest relative based on 16S rRNA gene sequence¶ | IAA productiona | ARAb | c P-solubilization | d K-solubilization | e S-production | Accession numbers |
---|---|---|---|---|---|---|---|---|---|---|---|
Leaf star | AF5 | Baghlan | Paddy | Leaf | Pseudomonas brassicacearum *(100%) | 8.4±0.2 | 2.9±0.1 | 0.0 | 0.0 | 6.0±0.8 | LC015569 |
AF19 | Baghlan | Paddy | Leaf | Rhizobium daejeonense (99%) | 10.9±0.3 | 2.7±0.2 | 0.0 | 0.0 | 3.0±0.2 | LC015583 | |
AF28 | Baghlan | Paddy | Leaf | Agrobacterium larrymoorei (99%) | 4.2±0.1 | 0.0 | 1.0±0.2 | 0.0 | 1.0±0.1 | LC015600 | |
AF113 | Baghlan | Paddy | Leaf | Acidovorax oryzae (100%) | 2.8±0.4 | 0.0 | 3.0±0.1 | 0.0 | 6.0±0.8 | LC015530 | |
AF52 | Takhar | Paddy | Root | Agrobacterium larrymoorei (99%) | 9.4±1.6 | 0.0 | 1.1±0.1 | 0.0 | 0.0 | LC015601 | |
AF71 | Badakhshan | Alfalfa | Root | Paenibacillus pabuli (99%) | 4.2±0.7 | 0.0 | 0.0 | 0.0 | 5.0±0.7 | LC015557 | |
AF90 | Kunduz | Paddy | Root | Variovorax paradoxus (100%) | 3.2±0.5 | 0.0 | 0.0 | 1.0±0.2 | 1.0±0.0 | LC015538 | |
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Sorkhaq | AF9 | Kunduz | Paddy | Leaf | Pantoea ananatis (100%) | 22.5±1.2 | 3.4±0.3 | 2.5±0.1 | 4.0±0.5 | 1.0±0.2 | LC015551 |
AF11 | Baghlan | Paddy | Leaf | Rhizobium rosettiformans (99%) | 11.7±2.2 | 0.5±0.0 | 0.0 | 0.0 | 0.0 | LC015582 | |
AF16 | Kunduz | Paddy | Leaf | Xanthomonas sacchari (99%) | 6.8±0.8 | 0.0 | 0.0 | 0.0 | 1.0±0.1 | LC015607 | |
AF22 | Badakhshan | Paddy | Leaf | Pseudomonas resinovorans (99%) | 3.9±0.9 | 0.2±0.0 | 0.0 | 0.0 | 0.0 | LC015561 | |
AF23 | Takhar | Paddy | Leaf | Brevundimonas bullata (99%) | 30.5±2.7 | 2.3±0.2 | 0.0 | 0.0 | 0.0 | LC015540 | |
AF84 | Baghlan | Paddy | Leaf | Rhizobium daejeonense (99%) | 17.4±2.6 | 629.1±23.8 | 0.0 | 0.0 | 0.0 | LC015590 | |
AF137 | Kabul | Clover | Leaf | Pseudomonas putida (100%) | 15.8±1.5 | 647.4±27.4 | 1.0±0.1 | 1.0±0.1 | 0.0 | LC015575 | |
AF42 | Badakhshan | Paddy | Root | Enterobacter ludwigii (99%) | 17.1±0.4 | 3.9±0.1 | 2.0±0.2 | 2.0±0.1 | 1.0±0.1 | LC015545 | |
AF74 | Badakhshan | Paddy | Root | Enterobacter ludwigii (99%) | 18.8±1.0 | 0.3±0.0 | 2.0±0.2 | 4.2±0.7 | 3.0±0.5 | LC015546 | |
AF91 | Baghlan | Paddy | Root | Paenibacillus bracinonensis (99%) | 0.0 | 0.3±0.0 | 1.5±0.3 | 0.0 | 0.0 | LC015555 | |
AF117 | Baghlan | Paddy | Root | Paenibacillus barcinonensis (99%) | 0.0 | 0.5±0.0 | 0.0 | 0.0 | 0.0 | LC015556 | |
AF135 | Kabul | Clover | Root | Enterobacter ludwigii (100%) | 74.9±4.7 | 0.5±0.0 | 1.5±0.4 | 0.0 | 2.0±0.3 | LC015542 | |
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Bala Doshi | AF100 | Takhar | Paddy | Leaf | Pseudomonas chengduensis (99%) | 0.0 | 0.0 | 0.0 | 0.0 | 6.0±0.7 | LC015559 |
AF51 | Takhar | Paddy | Leaf | Rhizobium daejeonense (99%) | 10.6±1.0 | 363.3±11.4 | 1.0±0.1 | 0.0 | 0.0 | LC015586 | |
AF69 | Takhar | Paddy | Leaf | Ensifer adhaerens (99%) | 2.0±1.0 | 0.0 | 0.0 | 0.0 | 0.0 | LC015581 | |
AF73 | Takhar | Paddy | Leaf | Pseudomonas putida (100%) | 7.4±0.8 | 14.1±1.1 | 1.5±0.4 | 0.0 | 6.5±0.4 | LC015579 | |
AF86 | Baghlan | Paddy | Leaf | Pseudomonas brassicacearum * (100%)10.0±2.4 | 0.8±0.0 | 0.0 | 0.0 | 6.0±0.9 | LC015572 | ||
AF6 | Baghlan | Paddy | Leaf | Agrobacterium tumefaciens (99%) | 16.5±0.9 | 0.0 | 1.0±0.3 | 0.0 | 0.0 | LC015597 | |
AF79 | Kunduz | Paddy | Leaf | Enterobacter ludwigii (99%) | 47.4±3.0 | 0.4±0.1 | 1.0±0.1 | 0.0 | 1.0±0.1 | LC015547 | |
AF30 | Takhar | Paddy | Root | Rhizobium daejeonense (99%) | 12.3±0.4 | 96.3±6.5 | 0.0 | 0.0 | 0.0 | LC015585 | |
AF32 | Kunduz | Paddy | Root | Enterobacter ludwigii (99%) | 23.6±2.6 | 0.0 | 1.3±0.4 | 4.0±0.9 | 0.0 | LC015544 | |
AF43 | Takhar | Paddy | Root | Pseudomonas mosselii (100%) | 7.6±0.8 | 0.0 | 5.0±0.6 | 0.0 | 3.0±0.5 | LC015563 | |
AF46 | Baghlan | Paddy | Root | Pseudomonas brassicacearum* (99%) | 3.1±1.8 | 16.0±0.9 | 0.0 | 0.0 | 6.0±0.3 | LC015571 | |
AF76 | Takhar | Paddy | Root | Pseudomonas putida (100%) | 9.9±1.4 | 1.0±0.1 | 0.0 | 0.0 | 7.0±0.9 | LC015578 | |
AF7 | Baghlan | Paddy | Root | Ralstonia insidiosa (100%) | 2.7±0.4 | 2.8±0.2 | 0.0 | 0.0 | 0.0 | LC015529 | |
AF8 | Takhar | Paddy | Root | Rhizobium borbori (99%) | 6.5±0.8 | 1.5±0.4 | 0.0 | 0.0 | 1.0±0.1 | LC015592 | |
AF130 | Baghlan | Paddy | Root | Brevundimonas diminuta (99%) | 3.2±1.8 | 1.2±0.0 | 0.0 | 0.0 | 0.0 | LC015539 | |
AF77 | Baghlan | Paddy | Root | N.D. | 2.1±0.6 | 0.2±0.0 | 0.9±0.2 | 0.0 | 0.0 | N.D. | |
AF96 | Takhar | Paddy | Root | Pseudomonas putida (100%) | 7.8±1.4 | 0.0 | 1.2±0.5 | 2.0±0.3 | 6.0±0.4 | LC015580 | |
AF99 | Kunduz | Paddy | Root | Pseudomonas oryzihabitans (99%) | 6.5±0.6 | 1.0±0.0 | 0.0 | 0.0 | 4.0±0.9 | LC015574 | |
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Look Andarab | AF54 | Badakhshan | Paddy | Leaf | Xanthomonas sacchari (99%) | 5.5±0.4 | 0.0 | 0.0 | 0.0 | 0.0 | LC015609 |
AF112 | Badakhshan | Alfalfa | Leaf | Pseudomonas plecoglossicida (99%) | 11.5±1.3 | 0.3±0.0 | 2.0±0.2 | 0.0 | 0.0 | LC015560 | |
AF40 | Takhar | Paddy | Root | Rhizobium borbori (99%) | 10.8±0.9 | 1.7±0.2 | 0.0 | 0.0 | 0.0 | LC015591 | |
AF75 | Badakhshan | Paddy | Root | Rhizobium daejeonense (99%) | 13.5±0.7 | 345.2±14.4 | 0.0 | 0.0 | 0.0 | LC015589 | |
AF124 | Badakhshan | Paddy | Root | Rhizobium daejeonense (99%) | 19.2±3.4 | 218.3±23.5 | 0.0 | 0.0 | 0.0 | LC015587 | |
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Monda Laghman | AF134 | Kabul | Clover | Leaf | Enterobacter ludwigii (99%) | 92.4±5.9 | 1.1±0.1 | 0.0 | 0.0 | 2.5±0.2 | LC015549 |
AF26 | Takhar | Paddy | Root | Agrobacterium tumefaciens (100%) | 8.6±0.5 | 0.0 | 0.0 | 1.0±0.2 | 0.0 | LC015595 | |
AF105 | Badakhshan | Paddy | Root | Enterobacter ludwigii (99%) | 32.3±2.0 | 1.2±0.1 | 0.0 | 0.0 | 0.0 | LC015541 | |
AF129 | Badakhshan | Paddy | Root | Pseudomonas brassicacearum* (99%) | 2.9±0.8 | 3.9±0.4 | 0.0 | 0.0 | 3.0±0.2 | LC015570 | |
AF29 | Badakhshan | Paddy | Root | Xanthomonas sacchari (99%) | 8.4±2.5 | 0.0 | 0.0 | 4.0±0.8 | 4.0±0.3 | LC015608 | |
AF26 | Takhar | Paddy | Root | Agrobacterium tumefaciens (100%) | 8.6±0.5 | 0.0 | 0.0 | 1.0±0.2 | 0.0 | LC015595 |
Amount of IAA produced (μg IAA per mL per 106 cells).
Acetylene reduction assay (ARA). Values represent activity expressed as nmol C2H4 h−1 10−6 cells.
P-solubilizing activity. Units represent size of clear zone (in mm) caused by dissolution of calcium phosphate.
K-solubilizing activity. Units represent size of clear zone (in mm) caused by dissolution of potassium mineral.
Siderophore production by bacterial strains. Units represent size of orange or yellow zone (in mm).
Pseudomonas brassicacearum subsp. Brassicacearum.
Values into parentheses indicate the percent of similarity between 16S RNA gene sequences of the isolates and those of known microorganisms of the NCBI GenBank.
N.D. means not determined.