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. Author manuscript; available in PMC: 2019 Dec 27.
Published in final edited form as: Cell. 2017 Mar 23;169(1):108–119.e20. doi: 10.1016/j.cell.2017.03.006

Figure 4. Receptor Clusters Increase the Probability of ZAP70 Recruitment to a DNA-Based CAR System Consisting of the Complete TCR.

Figure 4

(A) Schematic of a DNA-based CAR consisting of the complete TCR (DNA-CARTCR).

(B) Quantification of ZAP70-GFP recruitment to DNA-CARTCR at 0.1 ligands/μm2 (16-mer). Bar plot shows the percentage of single bound ligand receptors and clusters (black bars) and percentage of ZAP70 recruitment (gray bars). Mean ± SD from n = 8 cells.

(C) Quantification of ZAP70 dwell time at single bound 16-mer ligands (n = 42).

(D) A typical example of a transient recruitment of ZAP70-GFP to a single DNA-CARTCR. See also Figure S5C.

(E) Quantification of ZAP70-GFP recruitment to DNA-CARTCR at 1 ligands/μm2 (16-mer). Organization of bar plot same as shown (B). Mean ± SD from n = 3 cells.

(F) Distribution of ZAP70 dwell times at single bound receptors (n = 11) and receptor clusters (n = 30).

See also Figure S5.