Antibodies |
Anti-ScpB-His6 rabbit antiserum |
Gruber Lab |
COD003 |
Anti-Smc polyclonal rabbit antibody, affinity purified |
Gruber Lab |
COD008 |
Chemicals, Peptides, and Recombinant Proteins |
Adenosine Triphosphate (ATP) |
Sigma-Aldrich |
Cat#A6419-10G |
Benzonase |
Sigma-Aldrich |
Cat#A1014 |
Bis(malemido)ethane (BMOE) |
Thermo Scientific |
Cat#22323 |
Breathe-Easy |
Diversified Biotech |
Cat#BEM-1 |
BsaI |
New England Biolabs |
Cat#R0559L |
Certified Low Melt Agarose |
Bio-Rad Laboratories |
Cat#161-3111 |
Dynabeads Protein-G |
Life Technologies |
Cat#10004D |
Erythromycin |
AppliChem |
Cat#A2275,0005 |
GlycolBlue |
Ambion |
Cat#AM9515 |
HaloTag-Oregon Green Ligand |
Promega |
Cat#G2801 |
HaloTag-TMR Ligand |
Promega |
Cat#G8251 |
HisTrap 5 mL |
GE Healthcare |
Cat#17-5247-01 |
HiTrap Blue HP |
GE Healthcare |
Cat#17-0407-01 |
HiTrap Butyl HP |
GE Healthcare |
Cat#28-4110-01 |
HiTrap Q HP |
GE Healthcare |
Cat#17-1153-01 |
HiTrap Heparin HP |
GE Healthcare |
Cat#17-0407-01 |
HiLoad 16/60 S75 |
GE Healthcare |
Cat#28-9893-33 |
HiLoad 16/60 S200 pg |
GE Healthcare |
Cat#28989335 |
HiLoad 10/300 S200 GL |
GE Healthcare |
Cat#17-5175-01 |
Lysozyme From Chicken Egg White |
Sigma-Aldrich |
Cat#L6876-5G |
Lincomycin Hydrochloride Hydrate |
Sigma-Aldrich |
Cat#31727-250MG |
Mineral Oil |
Sigma-Aldrich |
Cat#M3516-1L |
Nicotinamide Adenine Dinucleotide (NADH) |
Santa Cruz |
Cat#205762A |
NuPAGE LDS Sample Buffer (4X) |
Life Technologies |
Cat#NP0008 |
Overnight Express Instant TB Medium |
Merck |
Cat#71491-5 |
Phosphoenolpyruvic Acid (PEP) |
Sigma-Aldrich |
Cat#P7002-100MG |
Phusion HotStart II DNA Polymerase |
Thermo Scientific |
Cat#F-549L |
Protease Inhibitor Cocktail |
Sigma-Aldrich |
Cat#P8849-5ML |
Pyruvate Kinase/Lactate Dehydrogenase |
Sigma-Aldrich |
Cat#P0294-5ML |
Ready-Lyse Lysozyme Solution |
Epicenter |
Cat#R1802M |
RNase A |
Sigma-Aldrich |
Cat#R5125 |
Sm DNase |
MPIB Core Facility |
SmDNase |
Superose 6 pg XK 16/70 |
GE Healthcare |
Cat#90-1000-42 |
Tris(2-carboxyethyl) Phosphine Hydrochloride Solution |
Sigma-Aldrich |
Cat#646547 |
T4 DNA Ligase |
Thermo Scientific |
Cat#EL0016 |
Critical Commercial Assays |
MasterBlock 96 well, 2 mL |
Greiner Bio One |
Cat#780270 |
Takyon SYBR 2xMasterMix Blue dTTP No ROX |
Eurogentec |
Cat#UF-NSMT-B0701 |
NuPAGE 4-12 % Bis-Tris Gels |
Life Technologies |
Cat#NP0323BOX |
Novex 4-12 % Tris-Glycine Mini Gels |
Life Technologies |
Cat#XP04125BOX |
NuPAGE 3-8 % Tris-Acetate Gels |
Life Technologies |
Cat#EA03755BOX |
QIAquick PCR Purification Kit |
QIAGEN |
Cat#28106 |
Costar Spin-X Centrifuge Tube Filter |
Corning |
Cat#8163 |
Ovation Ultralow System V2 |
NuGEN |
Cat#0344 |
Deposited Data |
Original data |
Mendeley Data |
DOI:10.17632/xzmvjdhwgt.1
|
ChIP-Seq Data |
NCBI Sequence Read Archive |
PRJNA533030 |
Experimental Models: Organisms/Strains |
E. coli and B. subtilis strains, see Table S6
|
Gruber Lab |
N/A |
Oligonucleotides |
yyaH Fw for qPCR CATATTGCCTTTTCCGTTGG |
Gruber Lab |
N/A |
yyaH rv for qPCR CATCCTCAATCACGCTTTCA |
Gruber Lab |
N/A |
parS-359 Fw for qPCR AAAAAGTGATTGCGGAGCAG |
Gruber Lab |
N/A |
parS-359 Rv for qPCR AGAACCGCATCTTTCACAGG |
Gruber Lab |
N/A |
Noc Fw for qPCR TCCATAATCGCCTCTTGGAC |
Gruber Lab |
N/A |
Noc Rv for qPCR AAGCGCATGCTTATGCTAGG |
Gruber Lab |
N/A |
yocGH Fw for qPCR TCCATATCCTCGCTCCTACG |
Gruber Lab |
N/A |
yocGH Rv for qPCR ATTCTGCTGATGTGCAATGG |
Gruber Lab |
N/A |
40 bp random sequence DNA A TTAGTTGTTCGTAGTGCTCGTCTGGCTCTGGATTAC CCGC |
Gruber Lab |
N/A |
40 bp random sequence DNA B GCGGGTAATCCAGAGCCAGACGAGCACTACGAACA ACTAA |
Gruber Lab |
N/A |
Recombinant DNA |
Plasmid DNA, see Table S7
|
Gruber Lab |
N/A |
Software and Algorithms |
Real Time PCR Miner |
Zhao and Fernald, 2005 |
http://ewindup.info/miner/ |
Bowtie2 Galaxy tool |
Langmead & Salzberg, 2012 |
https://usegalaxy.org |
GraphPad Prism 7.00 |
GraphPad Software |
www.graphpad.com |
ImageQuant TL 1D V8.1 |
GE Healthcare |
https://www.gelifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110 |
Seq-Monk |
Babraham Bioinformatics |
https://www.bioinformatics.babraham.ac.uk/projects/seqmonk/ |