| Antibodies |
| Anti-ScpB-His6 rabbit antiserum |
Gruber Lab |
COD003 |
| Anti-Smc polyclonal rabbit antibody, affinity purified |
Gruber Lab |
COD008 |
| Chemicals, Peptides, and Recombinant Proteins |
| Adenosine Triphosphate (ATP) |
Sigma-Aldrich |
Cat#A6419-10G |
| Benzonase |
Sigma-Aldrich |
Cat#A1014 |
| Bis(malemido)ethane (BMOE) |
Thermo Scientific |
Cat#22323 |
| Breathe-Easy |
Diversified Biotech |
Cat#BEM-1 |
| BsaI |
New England Biolabs |
Cat#R0559L |
| Certified Low Melt Agarose |
Bio-Rad Laboratories |
Cat#161-3111 |
| Dynabeads Protein-G |
Life Technologies |
Cat#10004D |
| Erythromycin |
AppliChem |
Cat#A2275,0005 |
| GlycolBlue |
Ambion |
Cat#AM9515 |
| HaloTag-Oregon Green Ligand |
Promega |
Cat#G2801 |
| HaloTag-TMR Ligand |
Promega |
Cat#G8251 |
| HisTrap 5 mL |
GE Healthcare |
Cat#17-5247-01 |
| HiTrap Blue HP |
GE Healthcare |
Cat#17-0407-01 |
| HiTrap Butyl HP |
GE Healthcare |
Cat#28-4110-01 |
| HiTrap Q HP |
GE Healthcare |
Cat#17-1153-01 |
| HiTrap Heparin HP |
GE Healthcare |
Cat#17-0407-01 |
| HiLoad 16/60 S75 |
GE Healthcare |
Cat#28-9893-33 |
| HiLoad 16/60 S200 pg |
GE Healthcare |
Cat#28989335 |
| HiLoad 10/300 S200 GL |
GE Healthcare |
Cat#17-5175-01 |
| Lysozyme From Chicken Egg White |
Sigma-Aldrich |
Cat#L6876-5G |
| Lincomycin Hydrochloride Hydrate |
Sigma-Aldrich |
Cat#31727-250MG |
| Mineral Oil |
Sigma-Aldrich |
Cat#M3516-1L |
| Nicotinamide Adenine Dinucleotide (NADH) |
Santa Cruz |
Cat#205762A |
| NuPAGE LDS Sample Buffer (4X) |
Life Technologies |
Cat#NP0008 |
| Overnight Express Instant TB Medium |
Merck |
Cat#71491-5 |
| Phosphoenolpyruvic Acid (PEP) |
Sigma-Aldrich |
Cat#P7002-100MG |
| Phusion HotStart II DNA Polymerase |
Thermo Scientific |
Cat#F-549L |
| Protease Inhibitor Cocktail |
Sigma-Aldrich |
Cat#P8849-5ML |
| Pyruvate Kinase/Lactate Dehydrogenase |
Sigma-Aldrich |
Cat#P0294-5ML |
| Ready-Lyse Lysozyme Solution |
Epicenter |
Cat#R1802M |
| RNase A |
Sigma-Aldrich |
Cat#R5125 |
| Sm DNase |
MPIB Core Facility |
SmDNase |
| Superose 6 pg XK 16/70 |
GE Healthcare |
Cat#90-1000-42 |
| Tris(2-carboxyethyl) Phosphine Hydrochloride Solution |
Sigma-Aldrich |
Cat#646547 |
| T4 DNA Ligase |
Thermo Scientific |
Cat#EL0016 |
| Critical Commercial Assays |
| MasterBlock 96 well, 2 mL |
Greiner Bio One |
Cat#780270 |
| Takyon SYBR 2xMasterMix Blue dTTP No ROX |
Eurogentec |
Cat#UF-NSMT-B0701 |
| NuPAGE 4-12 % Bis-Tris Gels |
Life Technologies |
Cat#NP0323BOX |
| Novex 4-12 % Tris-Glycine Mini Gels |
Life Technologies |
Cat#XP04125BOX |
| NuPAGE 3-8 % Tris-Acetate Gels |
Life Technologies |
Cat#EA03755BOX |
| QIAquick PCR Purification Kit |
QIAGEN |
Cat#28106 |
| Costar Spin-X Centrifuge Tube Filter |
Corning |
Cat#8163 |
| Ovation Ultralow System V2 |
NuGEN |
Cat#0344 |
| Deposited Data |
| Original data |
Mendeley Data |
DOI:10.17632/xzmvjdhwgt.1
|
| ChIP-Seq Data |
NCBI Sequence Read Archive |
PRJNA533030 |
| Experimental Models: Organisms/Strains |
| E. coli and B. subtilis strains, see Table S6
|
Gruber Lab |
N/A |
| Oligonucleotides |
| yyaH Fw for qPCR CATATTGCCTTTTCCGTTGG |
Gruber Lab |
N/A |
| yyaH rv for qPCR CATCCTCAATCACGCTTTCA |
Gruber Lab |
N/A |
| parS-359 Fw for qPCR AAAAAGTGATTGCGGAGCAG |
Gruber Lab |
N/A |
| parS-359 Rv for qPCR AGAACCGCATCTTTCACAGG |
Gruber Lab |
N/A |
| Noc Fw for qPCR TCCATAATCGCCTCTTGGAC |
Gruber Lab |
N/A |
| Noc Rv for qPCR AAGCGCATGCTTATGCTAGG |
Gruber Lab |
N/A |
| yocGH Fw for qPCR TCCATATCCTCGCTCCTACG |
Gruber Lab |
N/A |
| yocGH Rv for qPCR ATTCTGCTGATGTGCAATGG |
Gruber Lab |
N/A |
| 40 bp random sequence DNA A TTAGTTGTTCGTAGTGCTCGTCTGGCTCTGGATTAC CCGC |
Gruber Lab |
N/A |
| 40 bp random sequence DNA B GCGGGTAATCCAGAGCCAGACGAGCACTACGAACA ACTAA |
Gruber Lab |
N/A |
| Recombinant DNA |
| Plasmid DNA, see Table S7
|
Gruber Lab |
N/A |
| Software and Algorithms |
| Real Time PCR Miner |
Zhao and Fernald, 2005 |
http://ewindup.info/miner/ |
| Bowtie2 Galaxy tool |
Langmead & Salzberg, 2012 |
https://usegalaxy.org |
| GraphPad Prism 7.00 |
GraphPad Software |
www.graphpad.com |
| ImageQuant TL 1D V8.1 |
GE Healthcare |
https://www.gelifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110 |
| Seq-Monk |
Babraham Bioinformatics |
https://www.bioinformatics.babraham.ac.uk/projects/seqmonk/ |
