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. 2019 Dec 27;9:20000. doi: 10.1038/s41598-019-56591-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MSCs exhibited low apoptosis levels after UV-B irradiation. (a) Percentage of apoptotic cells assessed by sub-G1 population and caspase-3 activation in MSCs and HS68 at 24, 48 and 96 hours after irradiation with UV-B light. Data are shown as mean ± standard deviation, n = 3. (b) Relative β-galactosidase staining intensity after 25 mJ/cm² or 100 mJ/cm² UV-B irradiation. Representative images show β-galactosidase expression as cellular senescence marker 96 hours after UV-B treatment (scale bar 200 µm). *P < 0.05, **P < 0.01, ***P < 0.001. Data are mean ± standard deviation, n = 4.