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. 2019 Dec 27;9:19950. doi: 10.1038/s41598-019-56276-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of SB203580 treatment in fibronectin expression in 3D-grown cells. (A) Phosphorylation of p38MAPK and MAPKMAP2 increases in MDA-MB-231 parental and organotropic metastasis derivatives in 3D suspension culture for 48 hours. The same volume of the set of cell lysates were loaded into different SDS-page gels. The blots of phosphorylated MAPKAPK and p38MAPK were cropped from the same gel and the other blots were cropped from different gels. (B) SB203580 was added as serial concentrations in 3D culture media and changes in fibronectin protein expression were monitored after cells were cultivated for 48 hours in 3D. The same volume of the set of cell lysates was loaded into different gels and p-p38 MAPK and MAPKAPK were cropped from the same gel. The other blots were cropped from different gels. (C) MDA-MB-231 parental and LM2 cells were treated with the p38 MAPK inhibitor, SB203580 for 48 hours and formation of cell-spheroids was observed. Scale bar: 100 μm.