Figure 1.

BC1 inhibits efflux and shows increased accumulation of EtBr in colistin resistant clinical isolate of E. coli. (A) Mid log cells of colistin resistant E. coli isolate U3790 were de-energized with CCCP (100 µM) for 20 min. The cells were then harvested, washed and incubated with different benzochrome derivatives (16 µg/ml) along with EtBr for an hour. The cells were washed again and energized in buffer containing glucose. Efflux of EtBr was recorded for 25 min at 5 min intervals. (B) Mid log cells were harvested in buffer and allowed to incubate with EtBr for 30 min and BC derivatives were added at 16 µg/ml. Fluorescence was measured for a period of 25 min at 5 min intervals. The experiments were performed in triplicates.