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. 2019 Dec 25;9:19845. doi: 10.1038/s41598-019-56325-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BC1 perturbs membrane potential similar to CCCP. Disc3 was added to mid log cells of U3790 and fluorescence (Ex 622 nm and Em 670 nm) was recorded until plateau. 0.5% glucose was added and fluorescence was again monitored. BC1 and CCCP was added, which led to sudden decline in the fluorescence intensity. The experiment was performed in triplicates and the error bar represents standard error of the mean.