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. 2019 Dec 25;9:19845. doi: 10.1038/s41598-019-56325-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BC1 and Triad combination (colistin + salicylate + BC1) were non – toxic to zebrafish. Zebrafish were injected intramuscularly with (a) 0.5X (b) 1X (c) 2X MEC of BC1 (d) Colistin + Sal + BC1. Liver and brain tissues were isolated by dissection from euthanized fish after 48 h and tested for liver (alpha and beta naphthol) and brain (acetylcholine esterase) enzyme activities. The experiment was performed in triplicates and statistical analyses were performed using Student’s t-test.