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. 2019 Dec 25;9:19845. doi: 10.1038/s41598-019-56325-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Triad combination (colistin + salicylate + BC1) effectively reduces bacterial bioburden in infected zebrafish. 10⁵ CFU/ml of U3790 was injected intramuscularly in zebrafish. Different treatment combinations were administered 2–3 h post-infection. The zebrafish were sacrificed after 48 h and the colony counts (CFU/ml) from dissected muscle tissue was determined by plating the samples on agar plates. Experiment was performed in triplicates and statistical analyses were performed using Student’s t-test.