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. 2019 Dec 27;9:19890. doi: 10.1038/s41598-019-56535-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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hBD3-mediated autophagy suppression promoted intestinal epithelial cells migration. Migration ability of IEC-6 was determined by wound healing assay (A, scale bar, 200 μm) and transwell migration assay (C, scale bar, 200 μm), and then analyzed respectively (B,D). ATG7 knockdown efficiency of IEC-6 cells was assessed by western blotting (E) and evaluated detailedly. (F) Migration ability of IEC-6 transfected with ATG7 siRNA was detected by wound healing assay (H, scale bar, 200 μm) and subsequently compared with other groups (G) to confirm the effect of hBD3-mediated autophagy suppression on the migration of intestinal epithelial cells. Data was expressed as mean ± SD from three independent experiments and representative images were presented. **p < 0.01, ***p < 0.001, ^#p > 0.05.