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. 2019 Dec 27;9:20098. doi: 10.1038/s41598-019-56480-1

Figure 4.

Figure 4

The expression patterns of transcriptional factor genes controlling Lhb in anterior pituitary cells cultured in the absence and presence of GnRH. Changes in Pitx1, Egr1, and Nr5a1 expression in cells cultured for 96 h without GnRH (A) and in the presence of 10 nM GnRH, applied after 20 h culture in GnRH-free medium (B). Data points in A are mean ± SEM values from a representative experiment shown as relative units, and in B are normalized mean ± SEM values from four to nine experiments per time point, each done in quadruplicates. In all panels, asterisks indicate significant difference vs. zero time point controls.