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. 2019 Dec 27;9:20098. doi: 10.1038/s41598-019-56480-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of in vivo injection of a GnRH receptor agonist and antagonist on Lhb expression. Experiments were performed with prepubertal female and male rats. (A–D) Effects of injection of buserelin acetate, a GnRH receptor agonist (5 µg/0.4 ml per animal), cetrorelix acetate, a GnRH receptor antagonist (100 µg/0.4 ml per animals,) or solvent (0.4 ml PBS) intraperitoneally. Animals were sacrificed 0.5, 1, 1.5 and 2 h after injection (A) or 3, 6 and 9 h after injection (B–D). Values were normalized vs. Gapdh (A,B,D) or Rpl19 (C).