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. 2019 Dec 27;9:20098. doi: 10.1038/s41598-019-56480-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GnRH receptor agonist-stimulated LH secretion in vitro and in vivo. (A,B) Time-courses of GnRH-induced LH release (A) and depletion of intracellular LH content (B). After overnight incubation, cells were stimulated with 10 nM GnRH for 1–5 h. At the end of incubation periods, medium (A) and cell lysates (B) were collected for LH measurements. (C) Concentration-dependent effect of cycloheximide, a eukaryote protein synthesis inhibitor, on 10 nM GnRH-induced LH release following 4 h incubation (normalized values). Asterisks indicate significant differences vs. untreated cells. (D,E) In vivo effects of buserelin acetate on serum (D) and intrapituitary (E) LH. Data shown are mean ± SEM, N = 5 per time point. Panels D and E shows combined data from short-term and long-term treatment with buserelin acetate and control females (compare Figs. 6 and 7).