Table 2.
Expression stability ranking of reference genes in zebrafish brain across wild type and lepr mutant adults during the fasting-refeeding experiment.
| BestKeeper | geNorm | NormFinder | |||||
|---|---|---|---|---|---|---|---|
| Ranking | r values | Ranking | SD values | Ranking | M values | Ranking | S values |
| g6pd | 0.676 | g6pd | 0.237 | g6pd | 0.466 | g6pd | 0.124 |
| ef1a | 0.655 | rpl13 | 0.246 | actb2 | 0.489 | ef1a | 0.158 |
| actb2 | 0.626 | tmem50a | 0.255 | tmem50a | 0.493 | rpl13 | 0.158 |
| b2m | 0.615 | actb2 | 0.268 | rpl13 | 0.507 | actb2 | 0.161 |
| rpl13 | 0.604 | ube2a | 0.270 | ef1a | 0.524 | tmem50a | 0.169 |
| tmem50a | 0.531 | ef1a | 0.283 | ube2a | 0.537 | ube2a | 0.173 |
| ube2a | 0.404 | b2m | 0.418 | b2m | 0.625 | b2m | 0.237 |
| rplp0 | 0.375 | rplp0 | 0.577 | rplp0 | 0.842 | rplp0 | 0.316 |
Abbreviations: SD = standard deviation, r = Pearson product-moment correlation coefficient, S = stability value, M = mean value of stability.