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. 2019 Dec 27;9:19915. doi: 10.1038/s41598-019-56536-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of LUNAR1 suppresses the proliferation and invasion of colorectal cancer cells via IGF1 signalling. (A) Downregulation of LUNAR1 by siRNA verified by real-time PCR. The relative expression of LUNAR1 was analyzed using the 2^−ΔΔCt method. (B) The proliferation of cells detected by CCK-8 reagent. The data represent the results of three independent experiments. (C) Colony formation assay of three independent experiments. (D) The percentage of early apoptotic cells (Annexin V-positive and PI-negative) and late apoptotic cells (Annexin V-positive and PI-positive); the data represent the results of three independent experiments. (E) Migration and invasion assays; the counts of cells are presented as the mean values per field from at least five randomly-selected low-powered fields (×200) from three independent experiments (error bars: Means ± SDs). (F) IGF1R expression was decreased after LUNAR1 siRNA transfection, and increased after ectopically expressing vector containing IGF1R; (G) The proliferation of CRC cells detected by CCK-8 reagent indicated that ectopically IGF1R expression after LUNAR1 interference reversed the results of LUNAR1 siRNA transfection alone, the data represent the results of three independent experiments. (*P < 0.05, **P < 0.001).