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. 2019 Dec 27;9:20198. doi: 10.1038/s41598-019-55952-8

Figure 3.

Figure 3

Representative chromatogram of m/z 302.3042 in a biological sample (A) and sphinganine standard (B) at 10.13 min. Characteristic MS/MS spectra of m/z 302.3042 in a biological sample (C) and sphinganine standard at 10.13 min, and fragment interpretation (D). MS/MS spectra reveal the characteristic fragmentation pattern of sphinganine. Fragment ions at m/z 284 and 266 have previously been described as a single dehydration and a double dehydration, respectively. It should be noted that the single dehydration product is much more abundant than the double dehydration product, which is also a characteristic pattern.