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. 2019 Dec 27;9:19902. doi: 10.1038/s41598-019-55681-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR-RNP delivery in rice. (A) Hygromycin resistant albino rice regenerants. (B) Hygromycin resistant green rice regenerants. (C) Both top and bottom gels represent agarose gel electrophoresis showing PDS amplicon, numbers represent independent transformation lines; 1 kb, molecular weight marker; wt, wild type; neg, water control. (D) Percentages of albino and green regenerants produced by three different transformation platforms. (E) Mutations in T0 transgenic lines. Blue letters, target sequences in PDS exon 1; Red letters, PAM sequences; White letters in blue or red boxes, substitution mutations; Black letter with underscore, insertion mutations; Event marked with star, event with random DNA inserted at the target sites; black vertical lines, positions where random DNA inserted; yellow highlighted numbers, sizes of random DNA fragments; WT, wild type; HM, homozygous; BI, biallelic; HT, heterozygous.