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. 2019 Dec 27;9:20031. doi: 10.1038/s41598-019-56265-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Midsagittal sections were made from decalcified paraffin-embedded femurs at 2 to 4 weeks postoperatively (PO) and stained for quantitative analyses of osteoclasts. Tartrate-resistant acid phosphatase-positive osteoclasts (red colour) adjacent to the newly formed collagen fibre (light beige) were higher in number in bones harvested from groups C and PC than those from groups W and P. n = 5–6 per group. Black bar represents 200 μm. Green bar represents 50 μm. CIA; collagen-induced arthritis, P.g; Porphyromonas gingivalis administration.