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. 2019 Dec 27;9:20005. doi: 10.1038/s41598-019-56550-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transduction efficiency and cytokine production by human monocytes. Monocytes from healthy volunteers were infected for 24 h with YF 17D, NYVAC-KC, NYVAC-KC-GPN, NYVAC-KC-gp120, RV-Gag or RV-gp120TM (white bars: MOI 1; grey bars: MOI 10). (A) YF17D and RV RNA copies were quantified by RT-PCR. (B) p24 concentrations in cell culture supernatant were measured by electroluminescence. (C) IFNα, IFNβ, IL-6 and IL-8 concentrations in cell culture supernatants were measured by Luminex. LPS (10 ng/ml) was used as a positive control. Dots and bars represent individual data and mean, respectively.