Skip to main content
. 2019 Dec 27;9:19866. doi: 10.1038/s41598-019-56432-9

Figure 5.

Figure 5

Analysis of genes up-regulated in the thymus of mice from spaceflight and ground control experiments. (a) Volcano plots of RNA-seq data of TSG in MHU-1 and MHU-2. Significantly up- or down-regulated TSGs (P < 0.05, two-fold change) are indicated as red dots. (b) RNA-seq tracks of Aire gene. Upper track is MHU-1 data and lower track is MHU-2. Parentheses show average values of normalised gene expression with standard deviation. ***P < 0.001, and *P < 0.05 of FDR. (c) Immunohistochemical staining of thymic sections from GC, MG and AG mice with anti-Aire (green), UEA-1 -lectin (red) and anti-CD8α (blue). UEA-1 is a marker of mTECs, scalebars 100 µm. M and C in panels indicate medulla and cortex, respectively. Dotted lines indicate the border of medulla and cortex. (d) Immunohistochemical staining of thymic sections from GC, MG and AG mice with a combination of anti-Krt5 (green), UEA-1 (red) and CD8α (blue) (upper panels) and a combination of anti-Krt5 (green) and anti-Krt8 (red) (middle and lower panels). Part of MG panel is enlarged in a lower panel. Scalebars, 100 µm. M and C in panels indicate medulla and cortex, respectively. Dotted lines indicate the border of medulla and cortex. The medulla and cortex border of MG samples was difficult to define from these sections.