Table 1.
PCR primer sequences used for RT-PCR analysis.
| PCR product | Sequence (5′-3′) | Fragment size (bp) |
|---|---|---|
| OCT3/4 | F-ACCCCTGGTGCCGTGAA | 190 |
| R-GGCTGAATACCTTCCCAAATA | ||
| SOX2 | F-CAGCGCATGGACAGTTAC | 321 |
| R-GGAGTGGGAGGAAGAGGT | ||
| NANOG | F-AAAGGCAAACAACCCACT | 270 |
| R-GCTATTCTTCGGCCAGTT | ||
| LIN28 | F-GTTCGGCTTCCTGTCCAT | 122 |
| R-CTGCCTCACCCTCCTTCA | ||
| DPPB5 | F-CGGCTGCTGAAAGCCATTTT | 215 |
| R-AGTTTGAGCATCCCTCGCTC | ||
| TDGF1 | F-TCCTTCTACGGACGGAACTG | 140 |
| R-AGAAATGCCTGAGGAAAGCA | ||
| GAPDH | F-GAATGGGCAGCCGTTAGGAA | 414 |
| R-GACTCCACGACGTACTCAGC |
RT-PCR: reverse transcription polymerase chain reaction; F: Forward primer; R: reverse primer.