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. 2019 Dec 27;9:20176. doi: 10.1038/s41598-019-56658-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission electron microscopy (TEM) of renal cortex ablated with BH. (A,B) TEM images of intact renal tissue adjacent to the BH lesion demonstrating proximal renal tubules and small caliber blood vessel with lymphocyte (arrow). (C,D) BH lesions containing a slurry of homogenized cellular debris <0.1 μm in size (C), occasional loose collagen fibers (arrows), and echinocytes (D). (E,F) At the lesion border, the transition zone width was 20 microns from fully intact to fully destroyed tissue (top red bar/dashed lines). Within the zone, the cells (arrows) are significantly damaged, without vili but containing some organelles (bottom).