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. 2019 Dec 27;9:20047. doi: 10.1038/s41598-019-56377-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protocol for CSC enrichment, enumeration, sorting, and sequencing. An overview of CSC analysis in this study. The whole blood obtained from a sarcoma patient was enriched using autoMACS Pro Separator (Step 1). CSCs were fixed and stained with antibodies specific for sarcoma or WBCs (Step 2). CSC enumeration (Step 3) and sorting (Step 4) were carried out using the On‐chip Sort system. Genome sequencing was performed after WGA of CSCs. The permission for the usage of image of On-chip Sort was obtained from On-chip Biotechnologies.