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. 2019 Dec 27;9:19908. doi: 10.1038/s41598-019-56465-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nodule number per plant (a,b) and relative abundance of ureides (RAU) (c–f) at different soybean growth stages for the treatments: control (non-inoculated), seed inoculation at planting, and additional inoculations at V4 (fourth trifoliate) and R1 (beginning of flowering) in four environments (KS1, KS2, KS3, and KS4). Error bars indicate the standard deviation for each data point (mean). KS = Kansas.