Figure 6.

(a) Movement index analysis in presence and absence of doxycycline. Three L4 worms/well were placed onto NGM 6-well plates, fed with OP50 E. coli, and incubated at 25 °C for six days in presence of 0 or 100 μM of doxycycline from the L4 larval stage. Then the plates with adult worms and progenies were analyzed by INVAPP Paragon software. Three independent experiments were carried out and the results were plotted using GraphPad Prism (v6), ***p < 0.001 vs the untreated control (D76N β₂-m expressing nematodes) and °p < 0.5 vs. the ctrl worms according to one-way Anova. (b) Egg-synchronized control worms (smg-1) and D76N β₂-m expressing worms were placed at 23 °C into fresh NMG plates seeded with OP50 E. coli. At day 5 of adulthood body bends were scored in liquid. At least three independent assays were performed. Data are mean of number of body bends/min ± SEM; ***p < 0.001 vs the untreated control (D76N β₂-m expressing nematodes) and ****p < 0.0001 vs the ctrl worms, according to one-way ANOVA (N = 40 animals for each group). (c) Percentage of β₂-m in eluted fractions from gel filtration of D76N β₂-m expressing worms treated with 0 or 100 μM of doxycycline, is given as ratio of D76N β₂-m quantity of eluted fraction/soluble fraction starting material quantified from WB bands density related to the monomeric molecular weight (mean ± SEM, n = 2). Uncropped scans of immunoblots are shown in Supplementary Fig. S6. Density of the bands was determined by Image Studio Lite (LI-COR Biosciences).