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. 2019 Dec 27;9:19836. doi: 10.1038/s41598-019-56270-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCR amplification of HDR-HygB repair template integration locus spanning the cleavage sites of the in-vitro-assembled RNP complexes. Expected amplicon size for the RNP1175 (A) and RNP9269 (B) complexes was 1734 bp and 1619 bp, respectively. M = 1 kb ladder; 1–11 = F. proliferatum ∆FUM1 mutants resistant to hygromycin B; 12 = wild-type strain ITEM 7595.