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. 2019 Dec 27;9:20089. doi: 10.1038/s41598-019-56577-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gel permeation chromatography (Sephacryl S-100, 25 × 300 mm) profiles of midgut gland homogenate supernatants from control (a), Cd (b) and Zn-exposed Arion vulgaris (c), showing absorptions 254 nm (green line), as well as concentrations of Cd (dotted line) and Zn (black line), as specified in (a). Elution peaks of calibration standards (blue dextran, ≥100 kDa; myoglobin, 17.5 kDa; and vitamin B12, 1.4 kDa) are marked by inverted black triangles above the elution profiles. Fractions collected and pooled for subsequent purification (Fig. 4c) are indicated by brace and LMW Zn label.