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. 2019 Dec 27;9:20089. doi: 10.1038/s41598-019-56577-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HPLC separation profile with (a) NH₂-column of pooled zinc-containing low molecular weight fractions of gel chromatography showing absorptions at 254 nm (green line), Zn concentration (thick black line) and solvent gradient (thin black line), (b) with Superdex peptide 10/300 GL column of pooled zinc-containing fractions after amino column separation and vacuum concentration with Speedvac. Pink line corresponds to refractive index signal while black line is zinc concentration.