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. 2019 Dec 27;9:20221. doi: 10.1038/s41598-019-56725-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic relationships among SSV strains. The amino acid and nucleotide sequence alignment was performed using ClustalW software, with an Avian anativirus TW90A as the out-group. The genetic distance was calculated by Kimura’s two-parameter method. Phylogenetic trees with 1,000 bootstrap replicates were generated by the Neighbor-Joining method using MEGA (ver. 6) based on the aa sequence of VP1 (A) and the entire genome (B) of SSV, PSV, and TW90A. Scale bar indicates amino acid or nucleotide substitutions per site. Strain Cam13 strain is shown in bold italic letters. The genotype is tentatively named based on the findings of our present study.