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. 2019 Dec 27;9:20165. doi: 10.1038/s41598-019-56781-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of GluN1, GluN2A, and GluN2B siRNA on Ca²⁺ level after stimulation of primary mouse astrocytes with 10 nM VGVAPG peptide in primary mouse astrocytes; (A) after 15 min and (B) after 30 min of exposure. Each point represents the mean ± SD of three independent experiments, each of which comprised six replicates per treatment group. **P < 0.01 versus the control cells.