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. Author manuscript; available in PMC: 2020 Sep 19.
Published in final edited form as: Cell. 2019 Sep 19;179(1):251–267.e24. doi: 10.1016/j.cell.2019.08.013

Figure 7: H3.3 K27M Transcriptional Network and snATAC-seq Analysis.

Figure 7:

(A-B) t-SNE featureplots depicting cell type-specific upregulation NANOG, OCT4, SOX2, MYC target genes, and embryonic stem cell (ES)-associated gene sets and the underexpression of PRC2, SUZ12, EED, and H3K27-bound gene sets for human cells (A) and analogous genes/genesets in mouse (B).

C-D) Binary regulon activity and corresponding scRNA-seq featureplot for EZH2, BRCA1 (C; Filbin et al. dataset), Ezh2, and Brca1(D; K27M mouse dataset).

E) Schematic of snATAC-seq sample preparation

F) tSNE of sc- and snATAC datasets from P50, F) E18) and G) K27M mouse brains

I) K27M snATAC-identified MSigDB pathways

J) Genome browser alignments of snATAC-seq, scATAC-seq, and bulk ATAC-seq. *-Tumor MG is an overlaid(red/black) alignment of snATAC-seq microglial clusters captured with the K27M cells.