Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 27;9:20152. doi: 10.1038/s41598-019-56817-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

LKU4-CM induces browning of 3T3-L1 adipocytes. (A,B) Day 6 3T3-L1 adipocytes were incubated with or without LKU4-CM and GW9662 for 36 h, as indicated. The mRNA (A) and protein levels (B) of genes involved in adipocyte browning were analyzed by RT-qPCR and immunoblotting (Cropped blots were used), respectively, and the results were expressed as fold changes compared to the control. (C) Luciferase activity in HEK293T cells cotransfected with a reporter plasmid containing the Ucp1 promoter (pGL3-Ucp1-Luc) and the pcDNA3-PPARγ and pCMX-RXRα expression plasmids. After 12 h of transfection, cells were incubated with LKU4-CM, rosiglitazone (Rosi, 5.5 μM), and/or GW9662 for 24 h, as indicated. (D) Day 8 3T3-L1 adipocytes were incubated with control bacterial culture medium (con) or LKU4-CM for 36 h and the OCR was then measured in the basal condition and in the presence of oligomycin, FCCP, and rotenone/antimycin A, at the indicated time point. LKU4-CM vs LKU4-CM + GW9662; *P < 0.05, **P < 0.005.