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. 2019 Dec 27;9:19939. doi: 10.1038/s41598-019-56466-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ceramide regulation during zebrafish embryogenesis. (a) Zebrafish at 48 hpf, 4 dpf and 7 dpf. (b) Heatmap of ceramide content in 48 hpf, 4 dpf and 7 dpf larvae, demonstrating time-dependent reduction in the majority of d19:1 (highlighted in red) ceramides (see Table S3 for quantifications). (c) LCB distribution of all detected ceramides from 48 hpf, 4 dpf and 7 dpf larvae. n = 4 per group. Representative data from two independent experiments are shown. Additional verification of LCB distributions in 48 hpf embryos (n = 5 total) was performed via untargeted lipidomics (Supplemental Methods).