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. 2019 Dec 27;9:19961. doi: 10.1038/s41598-019-56509-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histology of freshly sliced (a) or cultivated (b) tumor-surrounding brain tissue (peritumoral tissue of zone III) and cultivated GBM tissue (d). Hematoxylin (nuclei) and eosin (cytoplasm) staining was done (a) instantly after the slicing procedure or after 13 days in culture (b,d). Apoptosis rate was determined by TUNEL staining in peritumoral tissue on day 0 (left bar) and day 13 (right bar) (c). Scale bar: 100 µm.