Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 27;9:19961. doi: 10.1038/s41598-019-56509-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Experimental setup. Freshly resected glioblastoma (zone I) and peritumoral brain tissue (zone III) was transported into the lab in sterile transport medium and stored at 4 °C. The production of 350 µm tissue slices was performed with a tissue chopper. The slices were separated from each other by the wide opening of a glass pipette and randomly allocated to membrane inserts and put in the wells of sterile 6-well plates, previously filled with 1 ml of cultivation medium. The slices were cultivated 10 days before treatment with radiochemotherapy was implemented. 24 hours prior to irradiation with 4 Gy the slices were pretreated with 200 µM temozolomide (TMZ). After a total treatment time of 72 hours the slices were either fixed in 4% paraformaldehyde for histological analyses or processed for RNA and protein isolation to perform whole transcriptome sequencing and protein analyses. We acknowledge Dr. Sonja Kallendrusch (Institute of Anatomy, University of Leipzig, Faculty of Medicine, Germany) who kindly provided the photograph of the tissue chopper.