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. 2019 Dec 27;9:19945. doi: 10.1038/s41598-019-56446-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Laconic reveals single-cell variations of stationary lactate levels. (a) Laconic FRET maps of a fat body, salivary gland, larval midgut (alkaline region, next to the midgut/hindgut junction) and larval brain (ventral nerve cord). In all four organs, cells with various different endogenous levels of lactate can be distinguished. In the brain sample, the specific area marked with a dotted line is analyzed in more detail in Supplementary Fig. 2. Scale bar: 50 μm. (b) The points represent the average FRET signal of each cell shown at the images of panel (a). Data distribution is represented by the box and whiskers graph.