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. 2019 Dec 27;9:20056. doi: 10.1038/s41598-019-56302-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of α-SMA is significantly upregulated in ECSC, and downregulated by ICG-001 and C-82. (A) The mRNA expression of α-SΜΑ was significantly upregulated in ECSC compared with NESC. n = 4. *p < 0.01, Student’s t-test. (B) mRNA expression of α-SΜΑ in ECSC was significantly downregulated by ICG-001 and C-82. n = 4. *p < 0.001, Student’s t-test. (C,D) Western blot analysis of α-SMA showed that protein expression of α-SMA was significantly upregulated in ECSC compared with NESC. n = 3. *p < 0.01, Student’s t-test. (E,F) Western blot analysis of α-SMA in control, ICG-001, and C-82 groups. There was no significant difference in the protein expression of α-SMA between the untreated control group and CBP/β-catenin inhibitor-treated groups by Student’s t-test. n = 4. Error bars represent standard deviation (SD). Uncropped images are shown in Figs. S6 and S7.