Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 27;9:19843. doi: 10.1038/s41598-019-56296-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Knocking down DST enhances YAP activity. (A) (Upper panels) Western blots on protein extracts from shLuc- or shDST-expressing MCF10A cells, blotted with anti-LATS1/2 (upper bands) or anti-GAPDH (lower bands). (Lower panels) Ratio of LATS1/2 levels between shLuc- and shDST-expressing MCF10A cells, normalized to GAPDH. Data are from three biological replicates. (B) Fold changes in CTGF or CYR61 or ITGB6 mRNA levels between shLuc- and shDST-expressing MCF10A cells. Data are from four biological replicates, performed in triplicates. (C) Fold changes in Luciferase activity between untreated MCF10A-shLuc cells and Tet-treated MCF10A-shDST cells, transfected with the YAP/TAZ-responsive MCAT-Luc reporter gene. Data are from three biological replicates, performed in triplicates. (D) (Left panels) Standard confocal sections of shLuc- or shDST-expressing MCF10A cells, stained with Phallodin (Magenta) to mark F-actin, anti-YAP (Yellow) and anti-Lamin (Blue) to mark the nuclear membrane. Scale bars represent 30 µm. (Right panel) quantifications of the percentage (%) of shLuc- or shDST-expressing MCF10A cells in which the ratio of YAP staining is higher in the nucleus than in the cytoplasm. Data are from three biological replicates. A total of 775 and 809 shLuc- and shDST-expressing cells, respectively, were quantified blind twice. (E) Fold changes in CTGF or CYR61 or ITGB6 mRNA levels in shLuc- or shDST-expressing MCF10A cells, treated with DMSO or Verteporfin (VP). Data are from three biological replicates, performed in triplicates. (F) Time course of growth rate for shLuc- or shDST-expressing MCF10A cells, before treatment (0 hours post-treatment) or treated with DMSO or VP for 24, 48 or 36 hours. A.U. Arbitrary Unit. Data are from three biological replicates. For all quantifications, error bars indicate SD.; ns indicates non-significant; *indicates P < 0.05; **indicates P < 0.005; ***indicates P < 0.001; ****indicates P < 0.0001.