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. 2019 Dec 27;9:19843. doi: 10.1038/s41598-019-56296-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpressing wts or knocking down yki suppresses the overgrowth of distal wing discs knocked down for shot. (A–F) standard confocal sections of third instar wing imaginal discs from females with dorsal up in which nub-Gal4 drives (A) UAS-GFP (green), UAS-mCherry (yellow) and UAS-RFP (yellow) or (B) two copies of UAS-GFP (green) and UAS-shot-RNAi or (C) UAS-mCherry (yellow), UAS-RFP (yellow) and UAS-Myc::wts (wts+) or (D) UAS-GFP (green), UAS-shot-RNAi and UAS-Myc::wts (wts+) or (E) UAS-GFP (green), UAS-mCherry (yellow) and UAS-yki-RNAi or (F) UAS-GFP (green), UAS-shot-RNAi and UAS-yki-RNAi. Discs are stained with anti-Disc large (Dlg) (magenta) to outline the disc area. The white lines outline the whole wing disc area. The yellow lines outline the nub > GFP- and/or nub > mCherry; RFP-expressing domains. Scale bars represent 100μm. (G,H) Quantification of the ratio between the GFP- or mCherry-expressing area and the total wing disc area in discs expressing the indicated UAS constructs under nub-Gal4. (G) 23 samples were analysed for nub > GFP, mCherry, RFP. 28 samples were analysed for nub > 2XGFP, shot-RNAi. 23 samples were analysed for nub > mCherry, RFP, wts⁺. 20 samples were analysed for nub > GFP, shot-RNAi, wts⁺. (H) 24 samples were analysed for nub > GFP, mCherry, RFP. 28 samples were analysed for nub > 2XGFP, shot-RNAi. 30 samples were analysed for nub > GFP, mCherry, yki-RNAi. 28 samples were analysed for nub > GFP, shot-RNAi, yki-RNAi. Error bars indicate SD. *indicates P < 0.05, **indicates P < 0.001, ****indicates P < 0.0001.