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. 2019 Dec 27;9:19843. doi: 10.1038/s41598-019-56296-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knocking down or overexpressing shot in the distal wing disc epithelium affects the expression of Yki target genes. All panels show standard confocal sections of third instar wing imaginal discs with posterior to the left and dorsal up in which hh-Gal4 drives (A-A″,B-B″) UAS-GFP (green in A,A′,B,B′) or (C-C″,D-D″) UAS-GFP (green in C,C″, D,D″) and UAS-shot-RNAi or (E-E″,F-F″) UAS-shotL(A)::GFP (green in E,E″,F,F″). Discs are stained with (A-A′,C-C′,E-E′) anti-Ex (magenta) or (B-B′,D-D′,F-F′) anti-β.galactosidade to reveal shg-LacZ expression (magenta). The white arrows in (D′ and F′) indicate the upregulation or downregulation, respectively, of shg::LacZ in cells flanking the dorsal-ventral boundary. Scale bars represent 50 μm.