Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 27;9:20257. doi: 10.1038/s41598-019-56208-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Reduced c-Cbl activity enhances tumor growth and immune infiltrates. (A) Average of tumor volumes from six mice injected subcutaneously with MC38 cells in c-Cbl^+/+ and c-Cbl^+/− groups and five in the c-Cbl^−/− group. All the c-Cbl^−/− mice had to be euthanized by 10–12 days with rapid development of the xenograft along with ulceration on the tumor. Student’s t-test was performed. Error bars = SEM. *p < 0.0001 corresponds to growth of tumors in c-Cbl^−/− mice compared to both c-Cbl^+/− and c-Cbl^+/+ groups. **p = 0.003 compares the group tumor growth c-between Cbl^+/− and c-Cbl^−/− group. (B) A representative mouse from three groups is shown at 12 days post-injection. (C) Excised xenografts are shown. Blue asterisk corresponds to the areas of necrosis and liquefaction (soft by palpation) compared to other areas of xenografts and yellow asterisk demarcates areas of ulceration. (D) Excised MC38 xenografts are shown. Representative images of a pair of c-Cbl^+/+ and c-Cbl^+/− mice are shown. (E) H&E stain of xenografts from different groups of mice are shown at 40X and 100X magnification. Xenografts from c-Cbl^+/+ and c-Cbl^+/− mice showed tumor cells (T) without evidence of acellular areas with fragmented nuclei suggestive of necrosis. Xenografts from c-Cbl^−/− mice showed acellular areas suggestive of necrosis (marked with a black asterisk) and several areas of pools of RBCs suggestive of hemorrhage (marked with a yellow asterisk). Scale bar = 25 micron (40×) and 50 micron (100×).