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Histological evaluation of printed constructs. Left: Representative images and Right: staining quantification of (a) alcian blue staining for sulfated glycosaminoglycans (GAG), (b) immunohistochemistry for type II collagen (COL II), and (c) immunohistochemistry for type I collagen (COL I) for printed constructs after 0, 28, and 56 days of culture or native bovine articular cartilage. Scale bars = 100 μm, n ≥ 15 sections, 45 images per group, *p < 0.05, **p < 0.01, ****p < 0.0001.
